mouse cxcl12 sdf 1 alpha quantikine elisa kit  (R&D Systems)

Journal: Apoptosis

Article Title: Spatially defined danger zone shapes gastric cancer progression through CCDC80 + fibroblast–induced CD8 + T cell dysfunction

doi: 10.1007/s10495-026-02287-1

Figure Lengend Snippet: CCDC80 + fibroblasts recruit effector CD8 + T cells into the danger zone and drive their exhaustion via CXCL12. a Spatial deconvolution of effector CD8 + T cells (CD8_eff) and exhausted CD8 + T cells (CD8_exhau). b Spatial CellChat analysis of CXCL signaling between CD8 + T cell subsets and CCDC80 + or CCDC80 − fibroblasts. c Bubble plot of ligand-receptor pairs between CD8 + T cell subsets and CCDC80 + or CCDC80 − fibroblasts. d Ligand–receptor interaction analysis showing exclusive engagement of CCDC80 + fibroblasts in CXCL12–CXCR4 signaling with both CD8_eff and CD8_exhau cells. e Quantification of CD8_eff and CD8_exhau recruitment in intestinal-type GC (GC3). f Spatial clustering of GC6 sample. g Pseudotime trajectory from cluster 1 to cluster 0. h Representative trajectory (15 − 10/38/62) demonstrating spatial progression to exhaustion. i qRT-PCR for CCDC80 mRNA expression in NIH-3T3 fibroblasts. j Immunoblot for CCDC80 protein expression. k qRT-PCR for CXCL12 mRNA in CCDC80-overexpressing fibroblasts. l ELISA for quantification of CXCL12 secretion. m Schematic of fibroblast-CD8 + T cell co-culture experiment. n Flow cytometry for PD-1 expression on CD8 + T cells with or without CXCL12 blockade. o Flow cytometry for TIM-3 expression on CD8 + T cells with or without CXCL12 blockade

Article Snippet: The collected supernatant was assayed for CXCL12 using an ELISA kit (Cat# MCX120, R&D Systems, Minneapolis, MN, USA), following the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Neutrophil depletion at the early stage of Japanese encephalitis virus infection affects CD8+ T cell infiltration into the mouse brain and causes severe encephalitis

doi: 10.3389/fimmu.2025.1748085

Figure Lengend Snippet: Chemokine levels in serum and brain lysates. (A) ELISA measured the concentration of CXCL12 (Top) and MIF-1 (bottom) levels in neutrophil-depleted mice, and AMD3100-treated infected mice serum (upper panel) and brain lysate (lower panel) plotted as a box plot. The concentration of MIF-1 level was measured using ELISA in neutrophil-depleted and AMD3100-treated infected mice serum (upper panel) and brain lysate (lower panel). (B) CXCL10 and CCL2 levels in serum (upper panel) and brain lysate (lower panel) in neutrophil-depleted mice and AMD3100-treated infected mice were measured by ELISA. The concentration of CXCL10 and CCL2 was plotted as a box plot. Each group has serum and brain lysate from (n=4) different infected mice. P - values were calculated using two-tailed unpaired Student’s t-test; *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: The serum and brain lysate from infected mice (JEV-S1 & JEV-S3) were used to detect CXCL12 (E-EL-M3046; Elabscience, Texas, USA), MIF-1 (EM0440, Fine Test, Broadway Street, US), CXCL10 (EM0004, Fine Test, Broadway Street, US), and CCL2 (EM0135, Fine Test, Broadway Street, US).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Infection, Two Tailed Test